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The objective of this study was to investigate whether a higher percentage of spermatozoa with proximal cytoplasmic droplets could affect the quality and fertilising ability of frozen-thawed semen of Bos indicus Nellore breed bulls, using routine laboratory sperm assays, plasma membrane, acrosome and mitochondrial status, chromatin integrity and IVF. Oocytes with at least four layers of cumulus cells were selected for the experiments.
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